Cart
Communication
A Photoactivatable Push−Pull Fluorophore for Single-Molecule Imaging in Live Cells
Full Text HTML
Hi-Res PDFPurchase the full-text
- PDF/HTML,
figures/images,
references and tables,
(where available)
Stanford University.
, ‡Kent State University.
Abstract
We have reengineered a red-emitting dicyanomethylenedihydrofuran push−pull fluorophore so that it is dark until photoactivated with a short burst of low-intensity violet light. Photoactivation of the dark fluorogen leads to conversion of an azide to an amine, which shifts the absorption to long wavelengths. After photoactivation, the fluorophore is bright and photostable enough to be imaged on the single-molecule level in living cells. This proof-of-principle demonstration provides a new class of bright photoactivatable fluorophores, as are needed for super-resolution imaging schemes that require active control of single molecule emission.
Citing Articles
Citation data is made available by participants in CrossRef's Cited-by Linking service. For a more comprehensive list of citations to this article, users are encouraged to perform a search in SciFinder.
This article has been cited by 12 ACS Journal articles (5 most recent appear below).
A New Highly Selective Fluorescent K+ Sensor
Xianfeng Zhou, Fengyu Su, Yanqing Tian, Cody Youngbull, Roger H. Johnson, and Deirdre R. MeldrumJournal of the American Chemical Society2011 133 (46), 18530-18533A New Highly Selective Fluorescent K+ Sensor
Xianfeng Zhou, Fengyu Su, Yanqing Tian, Cody Youngbull, Roger H. Johnson, and Deirdre R. MeldrumJournal of the American Chemical Society2011 133 (46), 18530-18533We describe the synthesis, properties, and application of a new fluorescent potassium chemosensor, KS2, for K+ sensing and imaging in live cells. By virtue of a strong electron-withdrawing group, 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran ...
In Situ Preparation of Highly Fluorescent Dyes upon Photoirradiation
Kakishi Uno, Hiroyuki Niikura, Masakazu Morimoto, Yukihide Ishibashi, Hiroshi Miyasaka, and Masahiro IrieJournal of the American Chemical Society2011 133 (34), 13558-13564In Situ Preparation of Highly Fluorescent Dyes upon Photoirradiation
Kakishi Uno, Hiroyuki Niikura, Masakazu Morimoto, Yukihide Ishibashi, Hiroshi Miyasaka, and Masahiro IrieJournal of the American Chemical Society2011 133 (34), 13558-13564Photoswitchable or photoactivatable fluorescent dyes are potentially applicable to ultrahigh density optical memory media as well as super-resolution fluorescence imaging when the dyes are highly fluorescent and have large absorption coefficients. Here, ...
Azido Push−Pull Fluorogens Photoactivate to Produce Bright Fluorescent Labels
Samuel J. Lord, Hsiao-lu D. Lee, Reichel Samuel, Ryan Weber, Na Liu, Nicholas R. Conley, Michael A. Thompson, Robert J. Twieg, and W. E. MoernerThe Journal of Physical Chemistry B2010 114 (45), 14157-14167Azido Push−Pull Fluorogens Photoactivate to Produce Bright Fluorescent Labels
Samuel J. Lord, Hsiao-lu D. Lee, Reichel Samuel, Ryan Weber, Na Liu, Nicholas R. Conley, Michael A. Thompson, Robert J. Twieg, and W. E. MoernerThe Journal of Physical Chemistry B2010 114 (45), 14157-14167Dark azido push−pull chromophores have the ability to be photoactivated to produce bright fluorescent labels suitable for single-molecule imaging. Upon illumination, the aryl azide functionality in the fluorogens participates in a photochemical conversion ...
Superresolution Imaging of Targeted Proteins in Fixed and Living Cells Using Photoactivatable Organic Fluorophores
Hsiao-lu D. Lee, Samuel J. Lord, Shigeki Iwanaga, Ke Zhan, Hexin Xie, Jarrod C. Williams, Hui Wang, Grant R. Bowman, Erin D. Goley, Lucy Shapiro, Robert J. Twieg, Jianghong Rao, and W. E. MoernerJournal of the American Chemical Society2010 132 (43), 15099-15101Superresolution Imaging of Targeted Proteins in Fixed and Living Cells Using Photoactivatable Organic Fluorophores
Hsiao-lu D. Lee, Samuel J. Lord, Shigeki Iwanaga, Ke Zhan, Hexin Xie, Jarrod C. Williams, Hui Wang, Grant R. Bowman, Erin D. Goley, Lucy Shapiro, Robert J. Twieg, Jianghong Rao, and W. E. MoernerJournal of the American Chemical Society2010 132 (43), 15099-15101Superresolution imaging techniques based on sequential imaging of sparse subsets of single molecules require fluorophores whose emission can be photoactivated or photoswitched. Because typical organic fluorophores can emit significantly more photons than ...
Visible Fluorescence Detection of Type III Protein Secretion from Bacterial Pathogens
Jacob S. Yount, Lun K. Tsou, Paul D. Dossa, Amy L. Kullas, Adrianus W. M. van der Velden and Howard C. HangJournal of the American Chemical Society2010 132 (24), 8244-8245Visible Fluorescence Detection of Type III Protein Secretion from Bacterial Pathogens
Jacob S. Yount, Lun K. Tsou, Paul D. Dossa, Amy L. Kullas, Adrianus W. M. van der Velden and Howard C. HangJournal of the American Chemical Society2010 132 (24), 8244-8245Type III protein secretion is essential for many Gram-negative bacterial infections of host cells and an attractive target for new antibacterial drugs. Here, we describe a bacterial protein effector-carboxypeptidase G2 (CPG2) reporter system for ...
Tools
-
Add to Favorites
-
Download Citation
-
Email a Colleague -
Permalink
Order Reprints
Rights & Permissions
Citation Alerts
History
- Published In Issue July 23, 2008
- Article ASAPJune 24, 2008
- Received: April 18, 2008
ACS
Network
Facebook
Tweet This
CiteULike
Newsvine
Digg This
Delicious
