Multiplexed Screening of Cellular Uptake of Gold Nanoparticles Using Laser Desorption/Ionization Mass Spectrometry

Zheng-Jiang Zhu, Partha S. Ghosh, Oscar R. Miranda, Richard W. Vachet* and Vincent M. Rotello*
Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003
J. Am. Chem. Soc., 2008, 130 (43), pp 14139–14143
DOI: 10.1021/ja805392f
Publication Date (Web): October 1, 2008
Copyright © 2008 American Chemical Society

Abstract

Abstract Image

Gold nanoparticles (AuNPs) are highly promising candidates as drug delivery agents into cells of interest. We describe for the first time the multiplexed analysis of nanoparticle uptake by cells using mass spectrometry. We demonstrate that the cellular uptake of functionalized gold nanoparticles with cationic or neutral surface ligands can be readily determined using laser desorption/ionization mass spectrometry of cell lysates. The surface ligands have “mass barcodes” that allow different nanoparticles to be simultaneously identified and quantified at levels as low as 30 pmol. Using this method, we find that subtle changes to AuNP surface functionalities can lead to measurable changes in cellular uptake propensities.

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History

  • Published In Issue October 29, 2008
  • Article ASAPOctober 01, 2008
  • Received: February 7, 2008

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