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Peptide-Based SAMs that Resist the Adsorption of Proteins

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Rolf Chelmowski^†, Stephan David Köster^‡, Andreas Kerstan^†, Andreas Prekelt^†, Christian Grunwald^§, Tobias Winkler, Nils Metzler-Nolte^‡, Andreas Terfort and Christof Wöll*^†

Physikalische Chemie 1 and Anorganische Chemie I, Ruhr-Universität Bochum, 44780 Bochum, Germany, Biochemie Biocenter, Johann Wolfgang Goethe-Universität, 60325 Frankfurt, Germany, and Anorganische Chemie, Phillips-Universität Marburg, 35032 Marburg, Germany

J. Am. Chem. Soc., 2008, 130 (45), pp 14952–14953

DOI: 10.1021/ja8065754

Publication Date (Web): October 17, 2008

, †

Physikalische Chemie 1, Ruhr-Universität Bochum.

, ‡

Anorganische Chemie I, Ruhr-Universität Bochum.

, §

Johann Wolfgang Goethe-Universität.

Phillips-Universität Marburg.

Section:

Biochemical Methods

Abstract

In this paper we present a modular approach for the fabrication of surfaces to characterize protein−protein interactions. The approach is based on azido peptides with an optimized sequence which are then thiol-functionalized using an alkynyl thiol and “click” chemistry. From these peptide thiols we fabricated SAMs on gold to evaluate the protein resistance, using surface plasmon resonance spectroscopy, toward streptavidin, bovin serum albumin (BSA), and fibronectin.

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This article has been cited by 9 ACS Journal articles (5 most recent appear below).

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Efficient Wastewater Treatment by Membranes through Constructing Tunable Antifouling Membrane Surfaces
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Patterning of Peptide Nucleic Acids Using Reactive Microcontact Printing
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- Patterning of Peptide Nucleic Acids Using Reactive Microcontact Printing
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  PNAs (peptide nucleic acids) have been immobilized onto surfaces in a fast, accurate way by employing reactive microcontact printing. Surfaces have been first modified with aldehyde groups to react with the amino end of the synthesized PNAs. When ...
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Salt Induced Irreversible Protein Adsorption with Extremely High Loadings on Electrospun Nanofibers
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  LiCl is a kosmotrope that generally promotes protein salvation in aqueous solutions. Herein we report that LiCl embedded in electrospun polymeric nanofibers interestingly induced an abnormal protein adsorption and substantially augmented the adsorption ...
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Achieving Highly Effective Nonfouling Performance for Surface-Grafted Poly(HPMA) via Atom-Transfer Radical Polymerization
Chao Zhao, Lingyan Li, and Jie Zheng
Langmuir2010 26 (22), 17375-17382
- Achieving Highly Effective Nonfouling Performance for Surface-Grafted Poly(HPMA) via Atom-Transfer Radical Polymerization
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  Human blood plasma and serum pose significant challenges to implanted devices because of highly unfavorable nonspecific protein adsorption on the surface. In this work, we introduce an improved two-step method to immobilize initiator thiols on a gold ...
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