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Fluorescein Redirects a Ruthenium−Octaarginine Conjugate to the Nucleus
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Section:Abstract
The cellular uptake and localization of a Ru−octaarginine conjugate with and without an appended fluorescein are compared. The inherent luminescence of the Ru(II) dipyridophenazine complex allows observation of its uptake without the addition of a fluorophore. Ru−octaarginine−fluorescein stains the cytosol, nuclei, and nucleoli of HeLa cells under conditions where the Ru−octaarginine conjugate without fluorescein shows only punctate cytoplasmic labeling. At higher concentrations, however, Ru−octaarginine without the fluorescein tag does exhibit cytoplasmic, nuclear, and nucleolar staining. Attaching fluorescein to Ru−octaarginine lowers the threshold concentration required for diffuse cytoplasmic labeling and nuclear entry. Hence, the localization of the fluorophore-bound peptide cannot serve as a proxy for that of the free peptide.
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This article has been cited by 9 ACS Journal articles (5 most recent appear below).
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Design and Synthesis of a New Class of Membrane-Permeable Triazaborolopyridinium Fluorescent Probes
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Correlation Between Cellular Localization and Binding Preference to RNA, DNA, and Phospholipid Membrane for Luminescent Ruthenium(II) Complexes
Maria Matson, Frida R. Svensson, Bengt Nordén, and Per LincolnThe Journal of Physical Chemistry B2011 115 (7), 1706-1711Correlation Between Cellular Localization and Binding Preference to RNA, DNA, and Phospholipid Membrane for Luminescent Ruthenium(II) Complexes
Maria Matson, Frida R. Svensson, Bengt Nordén, and Per LincolnThe Journal of Physical Chemistry B2011 115 (7), 1706-1711Because of their unique photophysical properties, sensitively depending on environment, ruthenium dipyridophenazine (dppz) complexes are interesting as probes for cellular imaging with fluorescence microscopy. Here three complexes derivatized with alkyl ...
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- Published In Issue July 01, 2009
- Article ASAPJune 08, 2009
- Received: March 30, 2009
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