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Tannin−Protein Complexes as Radical Scavengers and Radical Sinks
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Section:Abstract
The 2,2‘-azinobis(3-ethylbenzothiazoline 6-sulfonic acid) radical cation (ABTS•+) decolorization assay has been used to determine the antioxidant activity of the polyphenol epicatechin16 (4 → 8) catechin (procyanidin, PC) alone or in complex with the model proteins bovine serum albumin (BSA) or gelatin. PC had a molar antioxidant capacity of approximately 54, 92, or 108 radicals at pH values of 3.0, 4.9, or 7.4, respectively. Radical scavenging occurred via a rapid step followed by a slow step. Interaction with gelatin reduced the rate of rapid scavenging by 50% (PC−BSA mixtures reduced by 15%). Inhibition paralleled formation of precipitable PC−protein complexes over a range of protein/PC ratios. However, inhibition was virtually overcome in 10 min. Reaction with ABTS•+ converted the PC−protein complexes from a dissociable form to a form resistant to dissociation by strong denaturants such as SDS. This study demonstrates that PC is a potent ABTS•+ scavenger even when bound to protein and that the complexes may act as a radical sink within the gastrointestinal tract.
Keywords: Procyanidin; condensed tannin; flavonoid; oxidation; recalcitrant; antioxidant capacity; decolorization assay
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History
- Published In Issue October 15, 2001
- Received for review May 24, 2001. Revised manuscript received August 14, 2001. Accepted August 16, 2001. This work was supported by the National Cancer Institute.
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