Analysis of Volatiles from Spanish Honeys by Solid-Phase Microextraction and Gas Chromatography−Mass Spectrometry

Headspace solid-phase microextraction (SPME), followed by gas chromatography (GC)−mass spectrometry (MS) determination, has been used for the analysis of honey volatiles. Two SPME fibers were employed to study the composition of volatiles from various types of Spanish honeys. The best results were obtained with the Carboxen/PDMS fiber, using a homogenization time of 1 h at 70 °C and a sampling period of 30 min. A total of 35 compounds were detected, most of them identified by GC−MS and quantified using external standards. Differences in the composition of honey volatiles were obtained, and these results allowed the differentiation of honeys. However, further studies are necessary to confirm the utility of this technique as an alternative tool for the characterization of the floral origin of honeys.

Keywords: Honey; volatiles; solid-phase microextraction; gas chromatography; mass spectrometry