Simultaneous Quantitative Analysis of Maillard Reaction Precursors and Products by High-Performance Anion Exchange Chromatography

Tomas Davidek,†§ Nathalie Clety,† Stéphanie Devaud,‡ Fabien Robert,‡ and Imre Blank*‡
Centres R&D Nestl SAS, Amiens, P.O. Box 47, 80800 Aubigny, France, and Nestl Research Center, P.O. Box 44, 1000 Lausanne 26, Switzerland
J. Agric. Food Chem., 2003, 51 (25), pp 7259–7265
DOI: 10.1021/jf034794n
Publication Date (Web): November 7, 2003
Copyright © 2003 American Chemical Society

 Dedicated to Professor Dr. Werner Grosch on the occasion of his 70th birthday.

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†

 Centres R&D Nestlé SAS, Amiens.

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§

 Present address:  Nestlé Research Center.

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‡

 Nestlé Research Center.

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*

 Author to whom correspondence should be addressed (telephone +21/785-8607; fax +21/785-8554; e-mail imre.blank@rdls.nestle.com).

Abstract

A new analytical setup allowing the simultaneous analysis of precursors and products of the Maillard reaction is described. It is based on high-performance anion exchange chromatography with electrochemical (ECD) and diode array detectors (DAD) coupled in series. Chromatography and detection were optimized to permit simultaneous monitoring of compounds relevant to the Maillard reaction, such as the sugar, the amino acid, and the corresponding Amadori compound as well as the cyclic intermediates 5-(hydroxymethyl)-2-furaldehyde, maltol, and 2,3-dihydro-3,5-dihydroxy-6-methyl-4(H)-pyran-4-one. Separation was achieved on a CarboPac PA-1 column using a gradient of sodium acetate in aqueous sodium hydroxide. The Amadori compound, glucose, and glycine were monitored by an ECD operating in the integrated amperometry mode. The number of analyzed compounds was further increased by coupling the ECD with a DAD for the analysis of ultraviolet-active constituents. This method was successfully applied to model Maillard reaction mixtures based on glucose and glycine.

Keywords: Maillard reaction; Amadori compounds; quantitative analysis; anion exchange chromatography; integrated amperometry; simultaneous analysis

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History

  • Published In Issue December 03, 2003
  • Received for review July 17, 2003. Revised manuscript received September 28, 2003. Accepted October 5, 2003.

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