Linolenic Acid-Modified Chitosan for Formation of Self-Assembled Nanoparticles

Chen-Guang Liu,†‡ Kashappa Goud H. Desai,‡ Xi-Guang Chen,† and Hyun-Jin Park*‡
Life Science College, Ocean University of China, Qingdao 266003, People's Republic of China, and Graduate School of Biotechnology, Korea University, 1,5-Ka Anam-Dong, Sungbuk-ku, Seoul 136-701, Korea
J. Agric. Food Chem., 2005, 53 (2), pp 437–441
DOI: 10.1021/jf040188w
Publication Date (Web): December 21, 2004
Copyright © 2005 American Chemical Society
†

 Ocean University of China.

,
‡

 Korea University.

,
*

 To whom correspondence should be addressed. Tel:  82-2-3290-3450. Fax:  82-2-927-9028. E-mail:  hjpark@korea.ac.kr.

Abstract

Chitosan was modified by coupling with linolenic acid through the 1-ethyl-3-(3-dimethylaminopropyyl)carbodiimide-mediated reaction. The degree of substitution was measured by 1H NMR, and it was 1.8%, i.e., 1.8 linolenic acids group per 100 anhydroglucose units. The critical aggregation concentration (CAC) of the self-aggregate of hydrophobically modified chitosan was determined by measuring the fluorescence intensity of the pyrene as a fluorescent probe. The CAC value in phosphate-buffered saline (PBS) solution (pH 7.4) was 5 × 10-2 mg/mL. The average particle size of self-aggregates of hydrophobically modified chitosan in PBS solution (pH 7.4) was 210.8 nm with a unimodal size distribution ranging from 100 to 500 nm. A transmission electron microscopy study showed that the formation of near spherical shape nanoparticles had enough structural integrity. The loading ability of hydrophobically modified chitosan (LA-chitosan) was investigated by using bovine serum albumin (BSA) as a model protein. Self-aggregated nanoparticles exhibited an increased loading capacity (19.85 ± 0.04 to 37.57 ± 0.25%) with an increasing concentration of BSA (0.1−0.5 mg/mL).

Keywords: Chitosan; linolenic acid; nanoparticles; self-aggregates; BSA

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History

  • Published In Issue January 26, 2005
  • Received for review April 16, 2004. Revised manuscript received September 17, 2004. Accepted October 14, 2004. We acknowledge the National Natural Science Foundation of China (30370344) and KOSEF (1999-2-220-009-4) for financial support.

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