Preparation and Characterization of Nanoparticles Containing Trypsin Based on Hydrophobically Modified Chitosan

Chen-Guang Liu,†§ Kashappa Goud H. Desai,§ Xi-Guang Chen,† and Hyun-Jin Park*§
Life Science College, Ocean University of China, Qingdao 266003, People's Republic of China, and Graduate School of Biotechnology, Korea University, 1,5-Ka Anam-Dong, Sungbuk-ku, Seoul 136-701, Korea
J. Agric. Food Chem., 2005, 53 (5), pp 1728–1733
DOI: 10.1021/jf040304v
Publication Date (Web): February 11, 2005
Copyright © 2005 American Chemical Society
†

 Ocean University of China.

,
§

 Korea University.

,
*

 Corresponding author (e-mail hjpark@korea.ac.kr; telephone 82-2-3290-3450; fax 82-2-927-9028).

Abstract

Trypsin was immobilized on linolenic acid modified chitosan using glutaraldehyde (GA) as cross-linker, which was confirmed by Fourier transform infrared (FTIR) spectra. The chitosan nanoparticles containing trypsin (TR) can be prepared after the sonication of immobilized trypsin. The GA concentration affected both the enzyme activity of the nanoparticle and particle size. Results indicated that the activity of trypsin immobilized onto linolenic acid modified chitosan nanoparticles increased with increasing concentration of GA up to 0.07% (v/v) and then decreased with increasing amount of GA. On the other hand, particle size increased (from 523 to 1372 nm) with the increasing concentration of GA (from 0.03 to 0.1% v/v). The enzyme catalytic characteristics of nanoparticle solution were also studied. The results showed that the kinetic constant value (Km) of TR immobilized on nanoparticle (71.9 mg/mL) was higher than that of pure TR (50.2 mg/mL). However, the thermal stability and optimum temperature of TR immobilized on nanoparticles improved, which make it more attractive in the application aspect.

Keywords: Chitosan; nanoparticles; trypsin; hydrophobically modified chitosan

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History

  • Published In Issue March 09, 2005
  • Received for review June 23, 2004. Revised manuscript received October 26, 2004. Accepted November 4, 2004. This study was supported by a Korea University Grant.

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