Rapid Determination of Polyphenols and Vitamin C in Plant-Derived Products

Stephane Georgé,*† Pierre Brat,‡ Pascaline Alter,‡ and Marie J. Amiot§
Centre Technique de la Conservation et des Produits Agricoles (CTCPA), Site Agroparc, F-84911 Avignon Cedex 9, Centre de Coopration Internationale en Recherche Agronomique pour le Dveloppement (CIRAD), Dpartement FLHOR, TA50/16, F-34398 Montpellier Cedex 5, and Human Nutrition and Lipids, Facult de Mdecine, UMR U-476 INSERM (National Institute of Health and Medical Research)/U-1260 INRA (National Institute for Agronomic Research), 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
J. Agric. Food Chem., 2005, 53 (5), pp 1370–1373
DOI: 10.1021/jf048396b
Publication Date (Web): January 28, 2005
Copyright © 2005 American Chemical Society
*

 To whom correspondence should be addressed. Phone:  +33(0) 490841709. Fax:  +33(0) 490841726. E-mail:  sgeorge@ctcpa.org.

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†

 CTCPA.

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‡

 CIRAD.

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§

 UMR U-476 INSERM/U-1260 INRA.

Abstract

Polyphenols, widely spread in our diet by the consumption of plant food products, are commonly determined using Folin−Ciocalteu reagent that interacts with other different reducing nonphenolic substances and leads to an overestimation of polyphenol content. In this paper we report an optimized Folin−Ciocalteu method to specifically determine the contents of total polyphenols and vitamin C. After the optimal conditions for the colorimetric assay were set, solid-phase extraction (Oasis HLB (hydrophilic−lipophilic balance)) was carried out to eliminate the water-soluble reducing interferences including vitamin C. Colorimetric correction was thus performed by subtracting interfering substances contained in the water washing extract from the raw extract. Moreover, vitamin C present in the water washing extract can be destroyed by heating and thus colorimetrically deduced. This procedure was set up with synthetic solutions and validated on different extracts from fruit products.

Keywords: Polyphenols; vitamin C; Folin−Ciocalteu; colorimetry; solid-phase purification; fruit; puree; juice

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History

  • Published In Issue March 09, 2005
  • Received for review September 24, 2004. Accepted December 14, 2004. The present study was financially supported by the Ministère de la Recherche et de la Technologie (France) and by the Regional Council of Provence-Alpes-Cote d'Azur.

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