Prev. Article Next Article Table of Contents

Article

Event-Specific Real-Time Detection and Quantification of Genetically Modified Roundup Ready Soybean

Supporting Info

Chia-Chia Huang and Tzu-Ming Pan*

Institute of Microbiology and Biochemistry, National Taiwan University, Taipei, Taiwan 106

J. Agric. Food Chem., 2005, 53 (10), pp 3833–3839

DOI: 10.1021/jf048580x

Publication Date (Web): April 15, 2005

Address correspondence to this author at the Institute of Microbiology and Biochemistry, National Taiwan University, 1 Sec. 4, Roosevelt Rd., Taipei, Taiwan 106 (fax +886-2-23627044; telephone +886-2-23630231, ext. 3813; e-mail tmpan@ntu.edu.tw).

Abstract

The event-specific real-time detection and quantification of Roundup Ready soybean (RRS) using an ABI PRISM 7700 sequence detection system with light upon extension (LUX) primer was developed in this study. The event-specific primers were designed, targeting the junction of the RRS 5‘ integration site and the endogenous gene lectin1. Then, a standard reference plasmid was constructed that carried both of the targeted sequences for quantitative analysis. The detection limit of the LUX real-time PCR system was 0.05 ng of 100% RRS genomic DNA, which was equal to 20.5 copies. The range of quantification was from 0.1 to 100%. The sensitivity and range of quantification successfully met the requirement of the labeling rules in the European Union and Taiwan.

Keywords: Real-time PCR; light upon extension (LUX); genetically modified organisms (GMOs); RRS

View: Full Text HTML | Hi-Res PDF