Real-Time Polymerase Chain Reaction Quantification of the Transgenes for Roundup Ready Corn and Roundup Ready Soybean in Soil Samples

S. Lerat,†‡ L. S. England,† M. L. Vincent,† K. P. Pauls,*‡ C. J. Swanton,*‡ J. N. Klironomos,*§ and J. T. Trevors*†
Departments of Environmental Biology, of Plant Agriculture, and of Botany, University of Guelph, Guelph, Ontario, Canada N1G 2W1
J. Agric. Food Chem., 2005, 53 (5), pp 1337–1342
DOI: 10.1021/jf048830+
Publication Date (Web): February 10, 2005
Copyright © 2005 American Chemical Society
†

 Department of Environmental Biology.

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‡

 Department of Plant Agriculture.

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*

 To whom correspondence should be addressed. (K.P.P.) E-mail:  ppauls@uoguelph.ca. Phone:  (519) 824-4120 ext. 52460. (C.J.S.) E-mail:  cswanton@uoguelph.ca. Phone:  (519) 824-4120 ext. 53386. (J.N.K.) E-mail:  jklirono@uoguelph. Phone:  (519) 824-4120 ext. 56007. (J.T.T.) E-mail:  jtrevors@uoguelph.ca. Phone:  (519) 824-4120 ext. 53367. Fax:  (519) 824-0442.

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§

 Department of Botany.

Abstract

A method for quantification of recombinant DNA for Roundup Ready (RR) corn and RR soybean in soil samples is described. Soil DNA from experimental field samples was extracted using a soil DNA extraction kit with a modified protocol. For the detection and quantification of recombinant DNA of RR corn and RR soybean, a molecular beacon and two pairs of specific primers were designed to differentially target recombinant DNA in these two genetically modified crops. Soil DNA extracts were spiked with RR corn or RR soybean DNA, and recombinant DNA was quantified using real-time PCR with a molecular beacon. As few as one copy of RR corn genome or one copy of RR soybean genome was detected in the soil DNA extract.

Keywords: Agriculture; DNA; detection; environment; genetically modified; PCR amplification; persistence; soil; transgenic plants

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  • Published In Issue March 09, 2005
  • Received for review July 14, 2004. Revised manuscript received December 7, 2004. Accepted December 16, 2004. This research was supported by a team NSERC (Canada) strategic grant (no. 258065-02) to K.P.P., J.N.K, J.T.T., and C.J.S. Infrastructure support was also provided by the Canadian Foundation for Innovation and Ontario Challenge Fund.

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