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Article

Comparison of Chemical Characteristics of Three Soybean Cysteine Proteinase Inhibitors

S. Lalitha,^† Richard E. Shade,^‡ Larry M. Murdock,^‡ P. Michael Hasegawa,^§ Ray A. Bressan,^§ and S. Suzanne Nielsen*^#

974 Bidwell Avenue, Sunnyvale, California 94086; Department of Entomology, Purdue University, 901 West State Street, West Lafayette, Indiana 47907-2089; Department of Horticulture and Landscape Architecture, Purdue University, 625 Agriculture Mall Drive, West Lafayette, Indiana 47907-2010; and Department of Food Science, Purdue University, 745 Agriculture Mall Drive, West Lafayette, Indiana 47907-2009

J. Agric. Food Chem., 2005, 53 (5), pp 1591–1597

DOI: 10.1021/jf048959j

Publication Date (Web): February 9, 2005

†

974 Bidwell Ave., Sunnyvale, CA.

‡

Department of Entomology, Purdue University.

Department of Horticulture and Landscape Architecture, Purdue University.

Author to whom correspondence should be addressed (e-mail nielsens@purdue.edu).

Department of Food Science, Purdue University.

Abstract

Three recombinant soybean cysteine proteinase inhibitors (rSCPIs) L1, R1, and N2 were chemically characterized. These inhibitors have the potential to inhibit the growth and development of three major agricultural crop pests known to utilize cysteine proteinases (CPs) for protein digestion: Western corn rootworm, Colorado potato beetle, and cowpea weevil. Characterization data obtained show differences between the inhibitors and will be needed to consider the use of rSCPIs to create insect resistance in plants.

Keywords: Cysteine proteinase inhibitors; Western corn rootworm; Colorado potato beetle; cowpea weevil; insect digestive enzymes

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