Structural Characteristics of Purified β-Conglycinin from Soybeans Stored under Four Conditions

H. J. Hou and K. C. Chang*
Department of Cereal and Food Sciences, North Dakota State University, Fargo, North Dakota 58105
J. Agric. Food Chem., 2004, 52 (26), pp 7931–7937
DOI: 10.1021/jf049430p
Publication Date (Web): November 26, 2004
Copyright © 2004 American Chemical Society
*

 Author to whom correspondence should be addressed [telephone (701) 231-7485; fax (701) 231-6536; e-mail kow.chang@ndsu.nodak.edu].

Abstract

Four storage conditions including adverse conditions [84% relative humidity (RH), 30 °C], mild conditions (57% RH, 20 °C), cold conditions (4 °C), and uncontrolled ambient conditions were used for storing soybeans. The storage time was 9 months for the adverse conditions and 18 months for the other three conditions. β-Conglycinin was purified and characterized with respect to its molecular properties. After storage under the adverse conditions, β-conglycinin showed no significant changes in total sugar content, surface hydrophobicity, free SH and SS bonds, and amino acid composition within 6 months; however, it showed a significant decrease in surface hydrophobicity and a significant increase in total free SH and total SH including SS content after 6 months. Analysis of the secondary structure showed a significant increase in α-helix content, but a significant decrease in β-sheet content after 3 months. For the other three conditions, no significant changes occurred to the structures of β-conglycinin when compared to the control. The molecular mass of β-conglycinin remained in the range of 199−212 kDa for all conditions during the entire storage periods.

Keywords: Soybeans; β-conglycinin; 7S protein; storage; structure

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History

  • Published In Issue December 29, 2004
  • Received for review April 6, 2004. Revised manuscript received September 27, 2004. Accepted October 11, 2004. Presented at the IFT Annual Meeting, Chicago, IL, July 12−16, 2003. An NSF-ND Epscor Doctoral Fellowship Grant, USDACSREES-NRI CGP 2001-10853, the North Dakota Soybean Council, and NDSU Hatch Project 2707 and ND 1905 provided funding for this project.

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