The Apoptotic Effect of Green Tea (−)-Epigallocatechin Gallate on 3T3-L1 Preadipocytes Depends on the Cdk2 Pathway

Bo-Tsung Wu,†§ Pei-Fang Hung,†§ Hui-Chian Chen,§ Rong-Nan Huang,§ Hsin-Huei Chang,§ and Yung-Hsi Kao*§
Department of Life Science, College of Science, National Central University, Chung-Li City, Taoyuan, Taiwan 32054, and Brain Research Center, University System of Taiwan, Taipei, Taiwan 112
J. Agric. Food Chem., 2005, 53 (14), pp 5695–5701
DOI: 10.1021/jf050045p
Publication Date (Web): June 8, 2005
Copyright © 2005 American Chemical Society
†

 These authors contributed equally to this work.

,
§

 National Central University.

,

 University System of Taiwan.

,
*

 Author to whom correspondence should be addressed [telephone (886)-3-4260839; fax (886)-3-4228482; e-mail ykao@cc.ncu.edu.tw].

Abstract

This study was designed to investigate the effect of green tea catechins, especially (−)-epigallocatechin gallate (EGCG), on the apoptosis of 3T3-L1 preadipocytes. Preadipocyte apoptosis as indicated by formation of DNA fragments was induced by EGCG in dose-dependent manners. While EGCG was demonstrated to decrease Cdk2 expression and activity and increase caspase-3 activity, overexpression of Cdk2 and treatment with the caspase-3 inhibitor respectively prevented preadipocytes from induction of DNA fragmentation and caspase-3 activity by doses of 100−400 μM of EGCG. This suggests the Cdk2- and caspase-3-dependent apoptotic effects of EGCG. Moreover, EGCG was more effective than EC, ECG, and EGC in changing the apoptotic signals. Results of this study may relate to the mechanism by which EGCG modulates body weight.

Keywords: Green tea; epigallocatechin gallate; 3T3-L1 preadipocyte; cyclin-dependent kinase; caspase-3; apoptosis

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History

  • Published In Issue July 13, 2005
  • Received for review January 10, 2005. Revised manuscript received May 2, 2005. Accepted May 2, 2005. This work was supported by grants (NSC90-2311-B-008-002 and NSC91-2311-B-008-004) from the National Science Council, Taiwan, and the Brain Research Center, University System of Taiwan, Taiwan, to Y.-H.K.

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