Evaluation of Nonstarch Polysaccharides and Oligosaccharide Content of Different Soybean Varieties (Glycine max) by Near-Infrared Spectroscopy and Proteomics

Kristin Hollung,*† Margareth Øverland,‡ Milica Hrustić,§ Petar Sekulić,§ Jegor Miladinović,§ Harald Martens,† Bjørg Narum,† Stefan Sahlstrøm,† Mette Sørensen,‡ Trond Storebakken,‡ and Anders Skrede‡
Matforsk, The Norwegian Food Research Institute, Osloveien 1, N-1430 s, Norway, Aquaculture Protein Centre, Centre of Excellence, Post Office Box 5003, N-1430 s, Norway, and Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia and Montenegro
J. Agric. Food Chem., 2005, 53 (23), pp 9112–9121
DOI: 10.1021/jf051438r
Publication Date (Web): October 14, 2005
Copyright © 2005 American Chemical Society
*

 To whom correspondence should be addressed:  Matforsk, Osloveien 1, N-1430 Ås, Norway. Telephone:  +47-64-97-01-42. Fax:  +47-64-97-03-33. E-mail:  kristin.hollung@matforsk.no.

,
†

 The Norwegian Food Research Institute.

,
‡

 Aquaculture Protein Centre.

,
§

 Institute of Field and Vegetable Crops.

Abstract

A total of 832 samples of soybeans were screened by near-infrared (NIR) reflectance spectroscopy, to identify soybean samples with a lower content of oligosaccharides and nonstarch polysaccharides (NSP). Of these, 38 samples were identified on the basis of variation in protein content and agronomic value and submitted to high-resolution NIR spectroscopy. On the basis of the NIR data, 12 samples were further selected for chromatographic characterization of carbohydrate composition (mono-, di-, and oligosaccharides and NSP). Their soluble proteins were separated by two-dimensional gel electrophoresis (2DE). Using partial least-squares regression (PLSR), it was possible to predict the content of total NSP from the high-resolution NIR spectra, suggesting that NIR is a suitable and rapid nondestructive method to determine carbohydrate composition in soybeans. The 2DE analyses showed varying intensities of several proteins, including the glycinin G1 precursor. PLSR analysis showed a negative correlation between this protein and insoluble NSP and total uronic acid (UA).

Keywords: Near-infrared spectroscopy; soybean; Glycine max; carbohydrate composition; proteomics; nonstarch polysaccharides

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History

  • Published In Issue November 16, 2005
  • Received for review June 17, 2005. Revised manuscript received September 8, 2005. Accepted September 15, 2005.

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