Development of a Fast Analytical Method for the Determination of Sudan Dyes in Chili- and Curry-Containing Foodstuffs by High-Performance Liquid Chromatography−Photodiode Array Detection

Vanessa Cornet,* Yasmine Govaert, Goedele Moens, Joris Van Loco, and Jean-Marie Degroodt
Department of Pharmaco-bromatology, Food Section, Institute of Public Health, J. Wytsmanstreet 14, 1050 Brussels, Belgium
J. Agric. Food Chem., 2006, 54 (3), pp 639–644
DOI: 10.1021/jf0517391
Publication Date (Web): January 13, 2006
Copyright © 2006 American Chemical Society
*

 To whom correspondence should be addressed. Tel:  +32 2 642 51 25. Fax:  +32 2 642 56 91. E-mail:  vanessa.cornet@iph.fgov.be.

Abstract

A simple and fast analytical method for the determination of sudans I, II, III, and IV in chili- and curry-containing foodstuffs is described. These dyes are extracted from the samples with acetonitrile and analyzed by high-performance liquid chromatography coupled to a photodiode array detector. The chromatographic separation is carried out on a reverse phase C18 column with an isocratic mode using a mixture of acetonitrile and water. An “in-house” validation was achieved in chili- and curry-based sauces and powdered spices. Depending on the dye, limits of detection range from 0.2 to 0.5 mg/kg in sauces and from 1.5 to 2 mg/kg in spices. Limits of quantification are between 0.4 and 1 mg/kg in sauces and between 3 and 4 mg/kg in spices. Validation data show a good repeatability and within-lab reproducibility with relative standard deviations < 15%. The overall recoveries are in the range of 51−86% in sauces and in the range of 89−100% in powdered spices depending on the dye involved. Calibration curves are linear in the 0−5 mg/kg range for sauces and in the 0−20 mg/kg range for spices. The proposed method is specific and selective, allowing the analysis of over 20 samples per working day.

Keywords: Sudan dyes; chili- and curry-containing foodstuffs; HPLC-PDA; food safety

View: Full Text HTML | Hi-Res PDF

Tools

History

  • Published In Issue February 08, 2006
  • Received for review July 20, 2005. Revised manuscript received November 30, 2005. Accepted December 1, 2005. We thank the Belgian Federal Agency for the Safety of the Food Chain for its financial support.

Recommend & Share

Related Content

Other ACS content by these authors: