Apple Procyanidin Oligomers Absorption in Rats after Oral Administration:  Analysis of Procyanidins in Plasma Using the Porter Method and High-Performance Liquid Chromatography/Tandem Mass Spectrometry

Toshihiko Shoji,*† Saeko Masumoto,† Nina Moriichi,† Hiroshi Akiyama,‡ Tomomasa Kanda,† Yasuyuki Ohtake,† and Yukihiro Goda‡
Fundamental Research Laboratory, Asahi Breweries, Ltd., I-21, Midori 1-chome, Moriya-shi, Ibaraki 302-0106, Japan, and Division of Foods, National Institute of Health Sciences, 1-18-1, Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan
J. Agric. Food Chem., 2006, 54 (3), pp 884–892
DOI: 10.1021/jf052260b
Publication Date (Web): January 5, 2006
Copyright © 2006 American Chemical Society
*

 To whom correspondence should be addressed. Tel:  +81-297-46-1504. Fax:  +81-297-46-1506. E-mail:  toshihiko.shoji@asahibeer.co.jp.

,
†

 Asahi Breweries, Ltd.

,
‡

 National Institute of Health Sciences.

Abstract

In this study, we investigated the absorption of apple procyanidins, namely, apple condensed tannins (ACTs), in rats using the Porter method and high-performance liquid chromatography/tandem mass spectrometry. The apple procyanidin concentrations in the rat plasma reached a maximum 2 h after administration and decreased thereafter. To investigate the limits of the absorption of apple procyanidins in the polymerization degree, we administered the procyanidin oligomer fraction, which was separated from ACT using normal-phase chromatography according to the degree of polymerization. Procyanidins from each dimer to pentamer group were detected in the plasma by the Porter method. Moreover, by the study using reconstituted procyanidins, polymeric procyanidins influenced the absorption of procyanidin oligomers. These results suggest that ACTs are absorbed and directly involved in physiological functions in the rats.

Keywords: Absorption; apple procyanidins; HPLC/MS/MS; procyanidin dimers; procyanidin trimers

View: Full Text HTML | Hi-Res PDF

Tools

History

  • Published In Issue February 08, 2006
  • Received for review September 13, 2005. Revised manuscript received November 16, 2005. Accepted November 17, 2005. This work was partly supported by a grant from the Human Science, Japan.

Recommend & Share

Related Content

Other ACS content by these authors: