Prev. Article Next Article Table of Contents

Article

Electrofocusing of Methanolic Extracts for Identification of Individual Flavonol Biomolecules in Camellia Species

K. M. Mariya John,* Deepu Vijayan, P. R. Rahul, S. D. Joshi, R. Raj Kumar, and A. K. A. Mandal

Division of Plant Physiology, UPASI Tea Research Institute, Nirar Dam BPO, Valparai, 642 127, TN, India

J. Agric. Food Chem., 2006, 54 (8), pp 2828–2831

DOI: 10.1021/jf052713c

Publication Date (Web): March 23, 2006

Address correspondence to this author at the UPASI Tea Research Institute, Nirar Dam BPO, Valparai 642 127, Coimbatore Dt, Tamil Nadu, India (telephone +91 4253 235301; fax +91 4253 235302; e-mail johnupasi@rediffmail.com).

Abstract

An effort has been made to isolate individual catechin compounds from green tea leaves in their pure form by electrophoresis. In the present study total polyphenol extraction was carried out initially and estimated through spectrophotometric and HPLC methods. Extracted polyphenol was separated on 0.7% agarose gel and visualized at 360 nm. Fragmented individual compounds were gel eluted with methanol and confirmed as (−)-epigallocatechin (EGC), (−)-epicatechin (EC), (−)-epicatechin gallate (ECG), and (−)-epigallocatechin gallate (EGCG) by HPLC. The method developed describes a suitable method for the isolation of valuable molecules in tea.

Keywords: Polyphenol extract; total catechins; agarose gel electrophoresis; gel elution

View: Full Text HTML | Hi-Res PDF