Production of Ascorbyl Palmitate by Surfactant-Coated Lipase in Organic Media

Hsin-Ju Hsieh,† Giridhar R. Nair,‡ and Wen-Teng Wu*‡
Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan, and Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan
J. Agric. Food Chem., 2006, 54 (16), pp 5777–5781
DOI: 10.1021/jf060089d
Publication Date (Web): July 15, 2006
Copyright © 2006 American Chemical Society
†

 National Tsing Hua University.

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‡

 National Cheng Kung University.

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*

 Corresponding author (telephone +886-6-237-6734; fax +886-6-275-4228; e-mail wtwu@mail.ncku.edu.tw).

Abstract

The surface of a lipase from Burkholderia cepacia was coated with a nonionic surfactant, propylene glycol monostearate, and was used as a biocatalyst in the production of ascorbic acid in tert-butyl alcohol. The influence of various factors such as the type of surfactant, the pH of the buffer used for coating, the amount of surfactant in the coating, the organic solvent, and the temperature and molar ratio of the substrates used in the reaction on the conversion of ascorbyl palmitate were studied. After 24 h of reaction at 50 °C, a conversion of 47% was obtained using an ascorbic acid to palmitic acid molar ratio of 1:6. The native lipase showed only 6% conversion.

Keywords: Acylation; antioxidant; ascorbyl palmitate; surfactant-coated lipase

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History

  • Published In Issue August 09, 2006
  • Received for review January 11, 2006. Revised manuscript received June 8, 2006. Accepted June 13, 2006.

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