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Size-Exclusion Chromatography of Tea Tannins and Intercepting Potentials of Peptides for the Inhibition of Trypsin−Caseinolytic Activity by Tea Tannins

Naoya Kasai* and Genki Nakatsubo

Osaka Prefecture University, Research Group of Food Material Chemistry, Division of Applied Biological Chemistry, Graduate School of Life and Environmental Sciences, 1-1 Gakuen-cho, Sakai, Osaka 599-8531, Japan

J. Agric. Food Chem., 2006, 54 (14), pp 5149–5156

DOI: 10.1021/jf060435r

Publication Date (Web): June 15, 2006

To whom correspondence should be addressed: Osaka Prefecture University, Research Group of Food Chemistry, Division of Applied Biological Chemistry, Graduate School of Life and Environmental Sciences, 1-1 Gakuen-cho, Sakai, Osaka 599-8531, Japan. Telephone: +81-(0)72-254-9460. Fax: +81-(0)72−254-9921. E-mail: kasai@biochem.osakafu-u.ac.jp.

Abstract

Molecular-weight distribution and characterization of tea tannin were investigated by high-performance liquid chromatography and the equivalent preparative exclusion gel chromatography using Sephadex G-25. The characteristics of the fractions were studied regarding the amounts of terminal catechin, sugar, and gallic acid, the color reaction of the Folin−Chiocalteu reagent, the UV absorbance, and the inhibition activity for the trypsin−caseinolytic activity per weight. Furthermore, we investigated the intercepting activities of the inhibition by the amino acids, peptides, their analogues, poly(ethylene glycol)s (PEGs), and histatin 5 using the inhibition of trypsin−caseinolytic activity by tea. Arg, Lys, and their peptides had strong intercepting activities for the inhibition, but only a weak activity was detected in the Pro peptides or gelatin-like peptides of (Pro-Pro-Gly)_n (n = 5 or 10). The guanidyl group of Arg and the amino methylene group of Lys were important for the intercepting activity, but the activity was weakly dependent upon the peptide bond formation. The intercepting activity of the peptides or PEG exponentially increased with the number of polymerizations. Histatin 5 did not have a remarkably strong intercepting activity considering the peptide length. The activity of the synthetic histatin 5 in which all of the Lys and Arg were substituted by Ala was at the same level as histatin 5.

Keywords: Tea tannin; trypsin; arginine; lysine; histatin

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