Composition of the Volatile Fraction of Ocotea bofo Kunth (Lauraceae) Calyces by GC-MS and NMR Fingerprinting and Its Antimicrobial and Antioxidant Activity

Alessandra Guerrini,† Gianni Sacchetti,† Mariavittoria Muzzoli,† Gabriela Moreno Rueda,§ Alessandro Medici,† Elena Besco,# and Renato Bruni*
Dipartimento delle Risorse Naturali e Culturali, Universit degli Studi di Ferrara, Corso Ercole d'Este 32, 44100 Ferrara, Italy; Fundacion Chankuap, Soasty y Domingo Comin, Macas, Ecuador; Dipartimento di Scienze Farmaceutiche, Universit degli Studi di Ferrara, Via Fossato di Mortara 1719, 44100 Ferrara, Italy; and Dipartimento di Biologia Evolutiva e Funzionale, Sezione Biologia Vegetale e Orto Botanico, Universit degli Studi di Parma, Parco Area delle Scienze 11A, 43100 Parma, Italy
J. Agric. Food Chem., 2006, 54 (20), pp 7778–7788
DOI: 10.1021/jf0605493
Publication Date (Web): September 13, 2006
Copyright © 2006 American Chemical Society
†

 Dipartimento delle Risorse Naturali e Culturali, Università degli Studi di Ferrara.

,
§

 Fundacion Chankuap.

,
#

 Dipartimento di Scienze Farmaceutiche, Università degli Studi di Ferrara.

,
*

 Author to whom correspondence should be addressed (e-mail bruni@biol.unipr.it; fax 0039 0521 905403).

,

 Università degli Studi di Parma.

Abstract

The chemical composition of the essential oil obtained by steam distillation of the floral calyces of Ocotea bofo Kunth (Lauraceae) was studied by means of GC, GC-MS, and 1H, 13C, and bidimensional NMR (COSY, HSQC, HMBC). Twenty-five constituents were identified, and estragole (48.7%), α-phellandrene (19.6%) and sabinene (10.4%) were found to be the major components. Antimicrobial activity against six aerobic bacteria and five yeasts and antioxidant activity performed by photochemiluminescence (PCL), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and β-carotene bleaching assays are reported. The oil showed fair inhibiting properties against bacteria and a good inhibition against most yeasts. Its radical scavenging and chain-breaking antioxidant properties were comparable to or better than those provided by synthetic controls. Particular emphasis has been given to the use of NMR as a fast and reliable tool to discriminate O. bofo essential oil from other commercial anethole- and estragole-rich oils, namely, Illicium verum, Foeniculum vulgare, and Artemisia dracunculus.

Keywords: Ocotea bofo; essential oil; antimicrobial; antioxidant; NMR fingerprinting

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History

  • Published In Issue October 04, 2006
  • Received for review February 24, 2006. Revised manuscript received July 5, 2006. Accepted July 9, 2006.

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