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Article

Use of NIRS for Quantification of Mangiferin and Hesperidin Contents of Dried Green Honeybush (Cyclopia genistoides) Plant Material

Elizabeth Joubert,*^†^‡ Marena Manley,^‡ and Mariza Botha^‡

ARC Infruitec-Nietvoorbij, Private Bag X5026, Stellenbosch, 7599, South Africa, and Department of Food Science, Stellenbosch University, Private Bag X1, Matieland (Stellenbosch) 7602, South Africa

J. Agric. Food Chem., 2006, 54 (15), pp 5279–5283

DOI: 10.1021/jf060617l

Publication Date (Web): July 1, 2006

To whom correspondence should be addressed: Tel: +27 21 809 3444. Fax: +27 21 809 3592. E-mail: joubertL@arc.agric.za.

†

ARC Infruitec-Nietvoorbij.

‡

Stellenbosch University.

Abstract

Cyclopia genistoides, normally used for the preparation of an herbal tea, honeybush, is a good source of the bio-active compounds mangiferin and hesperidin and is in demand for the preparation of xanthone-enriched extracts. Near-infrared spectroscopy (NIRS) was used to develop calibration models to predict the mangiferin and hesperidin contents of the dried green plant material. NIRS measurements of plant material and pure compounds were performed in diffuse reflectance mode. The calibration sets for mangiferin and hesperidin contents ranged from 0.7 to 7.21 and 0.64−4.80 g/100 g, respectively. Using independent validation, it was shown that the NIRS calibration models for the prediction of mangiferin (SEP = 0.46 g/100 g; R² = 0.74; and RPD = 1.96) and hesperidin (SEP = 0.38 g/100 g; R² = 0.72; and RDP = 1.90) contents of the dried plant material are adequate for screening purposes, based on RPD values.

Keywords: Near-infrared spectroscopy; honeybush; Cyclopia genistoides; mangiferin; hesperidin; xanthone; flavanone

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History

Published In Issue July 26, 2006
Received for review March 3, 2006. Revised manuscript received May 5, 2006. Accepted May 23, 2006. Funding was supplied by the Raps Foundation, Germany, The Technology and Human Resource for Industry Program (THRIP), South Africa, and the National Research Foundation (NRF), South Africa. The NRF also supplied a student bursary for M.B.