Preparation of Glucosamine from Exoskeleton of Shrimp and Predicting Production Yield by Response Surface Methodology

Javid S. Mojarrad,*†‡ Mahboob Nemati,‡§ Hadi Valizadeh,‡ Masood Ansarin,‡ and Samira Bourbour†
Departments of Medicinal Chemistry, Pharmacognosy and Food Science, and Pharmaceutics, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran, and Food Science and Nutraceuticals Lab, Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
J. Agric. Food Chem., 2007, 55 (6), pp 2246–2250
DOI: 10.1021/jf062983a
Publication Date (Web): February 21, 2007
Copyright © 2007 American Chemical Society
*

 To whom correspondence should be addressed. Tel:  98-411-3341315. Fax:  98-411-3344798. E-mail:  shahbazi_j@tbzmed.ac.ir or jvshahbazi@yahoo.com.

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†

 Department of Medicinal Chemistry.

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‡

 Drug Applied Research Center.

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§

 Department of Pharmacognosy and Food Science.

,

 Department of Pharmaceutics.

Abstract

Chitin was prepared from Persian Gulf shrimp (Metapenaeus monoceros), and then, the obtained chitin was hydrolyzed by hydrochloric acid solutions. The production yield of glucosamine hydrochloride from chitin was optimized, and the effect of three factors (acid concentration, acid to chitin ratio, and reaction time) was investigated. A Box−Behnken design by Minitab software created 12 reactions with different conditions. Each reaction was performed in two replicates. Response surface methodology was used for predicting the glucosamine preparation. The optimum conditions for glucosamine hydrochloride preparation were 30 and 37% hydrochloric acid, 9:1 (v/w) acid solution to solid ratio, and 4 h of reaction time. Time ratio and time acid concentrations were the effective factors on the yield.

Keywords: Chitin; glucosamine; hydrolysis; response surface methodology

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History

  • Published In Issue March 21, 2007
  • Received for review October 17, 2006. Revised manuscript received December 19, 2006. Accepted December 26, 2006. We thank the Vice Chancellor for Research of Tabriz University of Medical Sciences for financial support of this research.

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