ACE-Inhibitory and Radical-Scavenging Activity of Peptides Derived from β-Lactoglobulin f(19−25). Interactions with Ascorbic Acid

Blanca Hernández-Ledesma, Lourdes Amigo, Isidra Recio, and Begoña Bartolomé*
Instituto de Fermentaciones Industriales (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain
J. Agric. Food Chem., 2007, 55 (9), pp 3392–3397
DOI: 10.1021/jf063427j
Publication Date (Web): April 6, 2007
Copyright © 2007 American Chemical Society
*

 To whom correspondence should be addressed. Tel.:  +34 91 5622900. Fax:  +34 91 5644853. E-mail:  bartolome@ifi.csic.es.

Abstract

In this work, the angiotensin-converting enzyme (ACE)-inhibitory and radical-scavenging activities of the β-lactoglobulin (β-Lg)-derived peptides WY f(19−20), WYS f(19−21), WYSL f(19−22), WYSLA f(19−23), WYSLAM f(19−24), and WYSLAMA f(19−25) have been determined. The ACE-inhibitory activity (IC50) varied from 38.3 to 90.4 μM, with the exception of WYS (>500 μM). All β-Lg-derived peptides also exhibited radical-scavenging activity (oxygen radical absorbance capacity (ORAC) values ranged from 4.45 to 7.67 μmol Trolox equivalents/μmol of peptide). The presence and position of amino acids Trp, Tyr, and Met were proposed to be responsible for the antioxidant activity. The equimolar amino acid mixtures of all the peptides showed ORAC values lower than those of the corresponding peptides, indicating that the peptidic bond or the structural conformation had a positive influence on this activity. Finally, positive antioxidant effects of WYS, WYSL, and WYLA with ascorbic acid were observed, whereas WY and WYSLAM showed negative effects, both cases for different molar ratio mixtures. These results should be taken into account in the development of new food ingredients on the basis of peptides from β-Lg.

Keywords: β-Lactoglobulin peptides; ACE-inhibitory activity; radical-scavenging activity; ORAC; ascorbic acid

View: Full Text HTML | Hi-Res PDF

Tools

History

  • Published In Issue May 02, 2007
  • Received for review November 27, 2006. Revised manuscript received February 16, 2007. Accepted March 7, 2007. This work has received financial support from the projects AGL 2003-01088 and CM-S0505- AGR-0153 (Comunidad de Madrid). B. Hernández-Ledesma was the recipient of a fellowship from Instituto Danone, Spain.

Recommend & Share

Related Content

Other ACS content by these authors: