Lipid Characterization of Mangrove Thraustochytrid − Schizochytrium mangrovei

King-Wai Fan,† Yue Jiang,*‡ Yun-Wing Faan,† and Feng Chen*†
Department of Botany, The University of Hong Kong, Pokfulam Road, Hong Kong, and Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong
J. Agric. Food Chem., 2007, 55 (8), pp 2906–2910
DOI: 10.1021/jf070058y
Publication Date (Web): March 24, 2007
Copyright © 2007 American Chemical Society
†

 The University of Hong Kong.

,
*

 Authors to whom correspondence should be addressed [(Y.J.) telephone 852-3411-7062; fax 852-3411-5995; e-mail yjiang@hkbu.edu.hk; (F.C.) telephone 852-2299-0309; fax 852-2299-0311; e-mail sfchen@hkusua.hku.hk].

,
‡

 Hong Kong Baptist University.

Abstract

Lipid class composition and distribution of fatty acids within the lipid pool of microalga, Schizochytrium mangrovei FB3 harvested at the late exponential phase, was studied, with special emphasis on the distribution of docosahexaenoic acid (C22:6 n-3, DHA). Neutral lipids were the major lipid constituents (95.90% of total lipids) in which triacylglyerol (TAG) was the predominant component and accounted for 97.20% of the neutral lipids. Phosphatidylcholine (PC) was the major polar lipid. Phosphatic acid and phosphatidylserine were the two classes in phospholipids reported for the first time in thraustochytrids. Both TAG and PC were primarily saturated and consisted of C16:0 at approximately 50% of total fatty acids. DHA was found to be distributed in all lipid classes and to be the major polyunsaturated fatty acid. TAG contained the highest amount of DHA, although the percentage of DHA in total fatty acids in TAG (29.74%) was lower than that in PC (39.61%). The result from this study would be useful for further optimization of DHA production by S. mangrovei.

Keywords: Lipid; docosahexaenoic acid (DHA); Schizochytrium mangrovei

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History

  • Published In Issue April 18, 2007
  • Received for review January 9, 2007. Revised manuscript received February 16, 2007. Accepted February 20, 2007. This research was supported by the RGC (Research Grants Council of Hong Kong).

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