Prev. Article Next Article Table of Contents

Article

Antioxidant Activity of Flavonoids Isolated from Young Green Barley Leaves toward Biological Lipid Samples

John A. Benedet, Hisao Umeda,^† and Takayuki Shibamoto*

Department of Environmental Toxicology, University of California, Davis, California 95616

J. Agric. Food Chem., 2007, 55 (14), pp 5499–5504

DOI: 10.1021/jf070543t

Publication Date (Web): June 1, 2007

†

Present address: Japan Pharmaceutical Development Co., Ltd., Umeda Yachiyo Blvd. 7th Floor, 2-22 Taiyujicho, Kita-ku, Osaka 530-0051, Japan.

To whom correspondence should be addressed. Address: Department of Environmental Toxicology, University of California, Davis, One Shields Avenue, Davis, California 95616. Phone: 530-752-4523. Fax: 530-752-3394. E-mail: tshibamoto@ucdavis.edu.

Abstract

Natural plant flavonoids, saponarin/lutonarin = 4.5/1, isolated from young green barley leaves were examined for their antioxidant activity using cod liver oil, ω-3 fatty acids, phospholipids, and blood plasma. The saponarin/lutonarin (S/L) mixture inhibited malonaldehyde (MA) formation from cod liver oil by 76.47 ± 0.11% at a level of 1 μmol and 85.88 ± 0.12% at a level of 8 μmol. The S/L mixture inhibited MA formation from the ω-3 fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) by 45.60 ± 1.08 and 69.24 ± 0.24%, respectively, at a level of 8 μmol. The S/L mixture inhibited MA formation from the phospholipids lecithin I and II by 43.08 ± 0.72 and 69.16 ± 2.92%, respectively, at a level of 8 μmol. It also inhibited MA formation from blood plasma by 62.20 ± 0.11% at a level of 8 μmol. The antioxidant activities obtained from the S/L mixture were comparable to those obtained from α-tocopherol and butylated hydroxy toluene (BHT) in all lipids tested.

Keywords: Antioxidant; blood plasma; cod liver oil; lutonarin; malonaldehyde; saponarin

View: Full Text HTML | Hi-Res PDF