Characterization of the Constituents and Antioxidant Activity of Brazilian Green Tea (Camellia sinensis var. assamica IAC-259 Cultivar) Extracts

Samuel T. Saito†, Grace Gosmann*†, Jenifer Saffi‡, Márcio Presser‡, Marc F. Richter‡ and Ana M. Bergold*†
Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Ipiranga 2752, Porto Alegre, RS, 90610-000, Brazil, and Laboratório de Genética Toxicológica, Universidade Luterana do Brasil, ULBRA, Canoas, RS, 92425-900, Brazil
J. Agric. Food Chem., 2007, 55 (23), pp 9409–9414
DOI: 10.1021/jf071796p
Publication Date (Web): October 16, 2007
Copyright © 2007 American Chemical Society
†

Universidade Federal do Rio Grande do Sul.

,
* To whom correspondence should be addressed. Phone: +55 51 3308-5451 . Fax: +55 51 3308-5313. E-mail: grace.gosmann@ufrgs.br (G.G.) or bergold@farmacia.ufrgs.br (A.M.B.).
,
‡

Universidade Luterana do Brasil.

Abstract

Freeze-dried extracts from Camellia sinensis var. assamica IAC-259 cultivar named Brazilian green tea were prepared by hot water and ultrasound-assisted extractions using leaves harvested in spring and summer. Their caffeine and catechin contents were measured by high performance liquid chromatography–diode array detector. The antioxidant activity of the major green tea compounds and Brazilian green tea extracts was evaluated using a 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The levels of caffeine were higher in the summer samples (p < 0.05); otherwise, there were no significant variations related to the catechin contents between spring and summer samples. The sonication method using water/acetone as solvent had a high efficiency to extract not only epigallocatechin gallate but also epicatechin gallate (p < 0.05). Antioxidant activities of the Brazilian green tea extracts were not significantly different among seasons and extraction systems. The antioxidant data (IC50) of the Brazilian green tea extracts showed a significant correlation with their epigallocatechin gallate and epicatechin gallate contents (p < 0.05).

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History

  • Published In Issue November 14, 2007
  • Article ASAPOctober 16, 2007
  • Received: June 18, 2007
    Accepted: September 11, 2007
    Revised: September 07, 2007

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