Phytoecdysteroids Increase Protein Synthesis in Skeletal Muscle Cells

Jonathan Gorelick-Feldman*†, David MacLean§, Nebojsa Ilic†, Alexander Poulev†, Mary Ann Lila#, Diana Cheng# and Ilya Raskin†
Biotech Center, Cook College, Rutgers University, 59 Dudley Road, New Brunswick, New Jersey 08901;Hallett Center for Diabetes and Endocrinology, Brown Medical School, Providence, Rhode Island 02912; and Department of Natural Resources and Environmental Sciences, University ofIllinois, Urbana, Illinois 61801
J. Agric. Food Chem., 2008, 56 (10), pp 3532–3537
DOI: 10.1021/jf073059z
Publication Date (Web): April 30, 2008
Copyright © 2008 American Chemical Society
* Author to whom correspondence should be addressed [e-mail jongf@eden.rutgers.edu; telephone (732) 932-8165, ext. 225 ; fax (732) 932-6535].
,
†

Rutgers University.

,
§

Brown Medical School.

,
#

University of Illinois.

Abstract

Phytoecdysteroids, which are structurally similar or identical to insect molting hormones, produce a range of effects in mammals, including increasing growth and physical performance. To study the mechanism of action of phytoecdysteroids in mammalian tissue, an in vitro cellular assay of protein synthesis was developed. In C2C12 murine myotubes and human primary myotubes, phytoecdysteroids increased protein synthesis by up to 20%. In vivo, ecdysteroids increased rat grip strength. Ecdysteroid-containing plant extracts produced similar results. The effect was inhibited by a phosphoinositide kinase-3 inhibitor, which suggests a PI3K-mediated mechanism.

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History

  • Published In Issue May 28, 2008
  • Article ASAPApril 30, 2008
  • Received: October 17, 2007
    Accepted: March 12, 2008
    Revised: February 14, 2008

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