Development of a Stable Isotope Dilution Assay for the Quantitation of Glycidamide and Its Application to Foods and Model Systems

Michael Granvogl#, Peter Koehler§, Larissa Latzer§ and Peter Schieberle*#§
Chair for Food Chemistry, Technical University of Munich, and German Research Center for Food Chemistry, Lichtenbergstrasse 4, D-85748 Garching, Germany
J. Agric. Food Chem., 2008, 56 (15), pp 6087–6092
DOI: 10.1021/jf800280b
Publication Date (Web): July 15, 2008
Copyright © 2008 American Chemical Society
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Technical University of Munich.

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§

German Research Center for Food Chemistry.

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* Corresponding author (telephone +49 89 289 132 65; fax +49 89 289 141 83; e-mail Peter.Schieberle@lrz.tum.de).

Abstract

On the basis of a stable isotope dilution assay and derivatization with 2-mercaptobenzoic acid, the presence of the carcinogenic glycidamide (2) in processed foods was verified for the first time. Using 13C-labeled 2 as the internal standard and the formation of the thioether derivatives, a new stable isotope dilution assay for the quantitation of 2 was developed. Application of the method on several potato samples revealed amounts between 0.3 and 1.5 μg/kg depending on the processing conditions. In a model experiment, the formation of 2 by an epoxidation of the double bond in acrylamide, that is, by a reaction with linoleic acid hydroperoxides, was established. This result was in good agreement with data showing that French fries processed in sunflower oil, which is high in linoleic acid, contained more 2 as compared to fries prepared in coconut oil. The derivatization procedure allows the simultaneous quantitation of acrylamide and glycidamide in foods.

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History

  • Published In Issue August 13, 2008
  • Article ASAPJuly 15, 2008
  • Received: January 27, 2008
    Accepted: May 20, 2008
    Revised: March 11, 2008

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