Prev. Article Next Article Table of Contents

Article

Brassinosteroids Promote Metabolism of Pesticides in Cucumber

Figures
Citing Articles

Your current credentials do not allow retrieval of the full text.

Purchase the full-text

PDF/HTML,
figures/images,
references and tables,
(where available)

Xiao Jian Xia†, Yun Zhang†, Jing Xue Wu†, Ji Tao Wang†, Yan Hong Zhou†, Kai Shi†, Yun Long Yu§ and Jing Quan Yu*†‡

^† Department of Horticulture, Zhejiang University, Kaixuan Road 268, Hangzhou 310029, China

^§ Department of Plant Protection, Zhejiang University, Hangzhou 310029, China

^‡ Key Laboratory of Horticultural Plants Growth, Development and Quality Improvement, Agricultural Ministry of China, Hangzhou 310029, China

J. Agric. Food Chem., 2009, 57 (18), pp 8406–8413

DOI: 10.1021/jf901915a

Publication Date (Web): August 20, 2009

*Corresponding author (telephone +86 571 86971120; fax +86 571 86049815; e-mail jqyu@zju.edu.cn).

Section:

Agrochemical Bioregulators

Abstract

Brassinosteroids (BRs) are known to protect crops from the toxicity of herbicides, fungicides and insecticides. It is shown here that application of 24-epibrassinolide (EBR) accelerated metabolism of various pesticides and consequently reduced their residual levels in cucumber (Cucumis sativus L). Chlorpyrifos, a widely used insecticide, caused significant reductions of net photosynthetic rate (Pn) and quantum yield of PSII (Φ_PSII) in cucumber leaves. EBR pretreatment alleviated the declines of Pn and Φ_PSII caused by chlorpyrifos application, and this effect of EBR was associated with reductions of chlorpyrifos residues. To understand how EBR promotes chlorpyrifos metabolism, the effects of EBR on activity and expression of enzymes involved in pesticide metabolism were analyzed. EBR had a positive effect on the activation of glutathione S-transferase (GST), peroxidase (POD), and glutathione reductase (GR) after treatment with chlorpyrifos, although the effect on GR was attenuated at later time points when plants were treated with 1 mM chlorpyrifos. In addition, EBR enhanced the expression of P450 and MRP, which encode P450 monooxygenase and ABC-type transporter, respectively. However, the expression of GST was consistently lower than that of plants treated with only chlorpyrifos. Importantly, the stimulatory effect of EBR on pesticide metabolism was also observed for cypermethrin, chlorothalonil, and carbendazim, which was attributed to the enhanced activity and genes involved in pesticide metabolism. The results suggest that BRs may be promising, environmentally friendly, natural substances suitable for wide application to reduce the risks of human and environment exposure to pesticides.

Keywords (keywords):

Brassinosteroids; Cucumis sativus; degradation; glutathione; enzyme

View: Full Text HTML | Hi-Res PDF | PDF w/ Links

Citing Articles

Citation data is made available by participants in CrossRef's Cited-by Linking service. For a more comprehensive list of citations to this article, users are encouraged to perform a search in SciFinder.

This article has been cited by 1 ACS Journal articles (1 most recent appear below).

Research Advances
Angela G. King
Journal of Chemical Education2010 87 (2), 123-127
- Research Advances
  Angela G. King
  Journal of Chemical Education2010 87 (2), 123-127
  The Research Advances for February 2010 summarizes research on how plant hormones called brassinosteroids can help to activate plant pesticide metabolism, the presence of a virus fighter in “Devil’s dung,” how kudzu isoflavones might improve human health, ...
  Abstract | Full Text HTML | Hi-Res PDF | PDF w/ Links