Analysis of the Polyphenols Content in Medicinal Plants Based on the Reduction of Cu(II)/Bicinchoninic Complexes

Daniele Cestari Marino, Larissa Zuppardo Lacerda Sabino, José Armando, Jr., Andrea De Andrade Ruggiero and Horacio Dorigan Moya*
Faculdade de Medicina da Fundação do ABC, CEPES (Centro de Estudos, Pesquisa, Prevenção e Tratamento em Saúde), Avenida Lauro Gomes, 2000, CEP 09060-650, Santo André, SP, Brazil
J. Agric. Food Chem., 2009, 57 (23), pp 11061–11066
DOI: 10.1021/jf902197p
Publication Date (Web): November 9, 2009
Copyright © 2009 American Chemical Society
*To whom correspondence should be addressed. Tel: +55 11 4993-7292. Fax: +55 11 4993-5410. E-mail: hdmoya@fmabc.br.
Biochemical Methods

Abstract

A spectrophotometric method is proposed for the determination of the polyphenols content in aqueous extracts of plants. The method is based on the reduction of Cu(II) to Cu(I) by polyphenols, in the presence of bicinchoninic acid in a buffered medium (ammonium acetate, pH 7.0) with the formation of Cu(I)/BCA complexes. A calibration curve of absorbance (at 558 nm) vs tannic acid concentration is linear (r = 0.995; n = 7) with tannic acid from 0.1 to 0.7 μmol L−1. The limit of detection and relative standard deviation were 40 nmol L−1 (99% confidence level) and 3.8% (0.4 μmol L−1 tannic acid, n = 7), respectively. For the aqueous extracts of Hamamelis virginiana L., Maytenus ilicifolia Mart. ex Reissek, Hydrocotyle bonariensis Lam, Annona muricata L., Myrciaria cauliflora (Mart.) O. Berg., Caesearia sylvestris Sw., Schinus terebinthifolia (Raddi), and Stryphnodendron adstringens (Mart.) Coville, the total polyphenol contents, expressed as tannic acid, were 3.5, 1.3, 2.0, 3.1, 15.4, 3.1, 9.1, and 6.9%, respectively.

Keywords (keywords):

Polyphenol determination; copper; 4,4′-dicarboxy-2,2′-biquinoline acid
View: Full Text HTML | Hi-Res PDF | PDF w/ Links

Tools

SciFinder Links

SciFinder subscribers: Click to sign in | Not a SciFinder subscriber? Learn more at www.cas.org

Explore by:

History

  • Published In Issue December 09, 2009
  • Article ASAPNovember 09, 2009
  • Received: December 09, 2008
    Revised: October 16, 2009
    Accepted: October 20, 2009

Recommend & Share

  • Share on ACS NetworkACS Network
  • Add to FacebookFacebook
  • Tweet ThisTweet This
  • Add to CiteULikeCiteULike
  • Add to NewsvineNewsvine
  • Digg ThisDigg This
  • Add to DeliciousDelicious

Related Content

Other ACS content by these authors: