Biocatalytic Microcontact Printing

Phillip W. Snyder,† Matthew S. Johannes,‡ Briana N. Vogen,† Robert L. Clark,*‡ and Eric J. Toone*†
Department of Chemistry, Duke University, Durham, North Carolina, 27708-0346, and The Pratt School of Engineering, Duke University, Durham, North Carolina, 27708-0271 rclark@egr.duke.edu; eric.toone@duke.edu
J. Org. Chem., 2007, 72 (19), pp 7459–7461
DOI: 10.1021/jo0711541
Publication Date (Web): August 18, 2007
Copyright © 2007 American Chemical Society
†

 Department of Chemistry.

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‡

 The Pratt School of Engineering.

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*

In papers with more than one author, the asterisk indicates the name of the author to whom inquiries about the paper should be addressed.

Abstract

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Immobilized biocatalytic lithography is presented as an application of soft lithography. In traditional microcontact printing, diffusion limits resolution of pattern transfer. By using an immobilized catalyst, the lateral resolution of microcontact printing would depend only on the length and flexibility of the tether (<2 nm) as opposed to diffusion (>100 nm). In the work, exonuclease reversibly immobilized on a relief-patterned stamp is used to ablate ssDNA monolayers Percent of ablation was determined via confocal fluorescence microscopy to be 70%.

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History

  • Published In Issue September 14, 2007
  • Received June 7, 2007

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