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A Photolabile Linker for the Mild and Selective Cleavage of Enriched Biomolecules from Solid Support

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Ronald Orth and Stephan A. Sieber

Center for Integrated Protein Science Munich CIPS, Department of Chemistry and Biochemistry, Ludwig-Maximilians-Universit

t M

nchen, Butenandtstrasse 5-13, 81377 Munich, Germany

J. Org. Chem., 2009, 74 (21), pp 8476–8479

DOI: 10.1021/jo901809k

Publication Date (Web): October 12, 2009

stephan.sieber@cup.uni-muenchen.de

Abstract

Selective release of enriched biomolecules from solid support is a desirable goal in proteomic and metabolomic studies. Here we demonstrate that photocleavage of a light-sensitive phenacyl ester bond is a suitable alternative cleavage strategy for the selective release of enriched biomolecules form avidin beads circumventing the disadvantages of conventional heat denaturation procedures.

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Published In Issue November 06, 2009
Article ASAPOctober 12, 2009
Received: August 21, 2009

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A Photolabile Linker for the Mild and Selective Cleavage of Enriched Biomolecules from Solid Support

Abstract

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SciFinder Links

History

Recommend & Share

Related Content

On the Selective N-Methylation of BOC-Protected Amino Acids

Synthesis of (S)- and (R)-5-Oxo-piperazine-2-Carboxylic Acid and Its Application to Peptidomimetics

How the Substituent at O-3 of N-Acetylglucosamine Impacts Glycosylation at O-4: A Comparative Study

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