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Article

Joint Statistical Analysis of Multichannel Time Series from Single Quantum Dot−(Cy5)_n Constructs^†

C. Shan Xu,^‡ Hahkjoon Kim,^§ Carl C. Hayden,*^§ and Haw Yang*^‡

Department of Chemistry, University of California at Berkeley, and Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, and Combustion Research Facility, Sandia National Laboratories, P.O. Box 969, Livermore, California 94551-0969

J. Phys. Chem. B, 2008, 112 (19), pp 5917–5923

DOI: 10.1021/jp075642o

Publication Date (Web): December 21, 2007

†

Part of the “Attila Szabo Festschrift”.

‡

University of California at Berkeley.

Sandia National Laboratories.

To whom correspondence should be addressed. E-mail: cchayde@ sandia.gov (C.C.H.); hawyang@berkeley.edu (H.Y.).

Lawrence Berkeley National Laboratory.

Abstract

One of the major challenges in single-molecule studies is how to extract reliable information from the inevitably noisy data. Here, we demonstrate the unique capabilities of multichannel joint statistical analysis of multispectral time series using Föster resonance energy transfer (FRET) in single quantum dot (QD)−organic dye hybrids as a model system. The multispectral photon-by-photon registration allows model-free determination of intensity change points of the donor and acceptor channels independently. The subsequent joint analysis of these change points gives high-confidence assignments of acceptor photobleaching events despite the interference from background noise and from intermittent blinking of the QD donors and acceptors themselves. Finally, the excited-state lifetimes of donors and acceptors are calculated using the joint maximum likelihood estimation (MLE) method on the donor and acceptor decay profiles, guided by a four-state kinetics model.

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History

Published In Issue May 15, 2008
Received July 18, 2007
Revised October 2, 2007

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Article

Joint Statistical Analysis of Multichannel Time Series from Single Quantum Dot−(Cy5)_n Constructs^†