Letter
Spontaneous Transfer of Phospholipid-Coated Oil-in-Oil and Water-in-Oil Micro-Droplets through an Oil/Water Interface
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Abstract

We studied the evolution of oil-in-oil (O/O) and water-in-oil (W/O) phospholipid-coated micro-droplets at an oil/water interface. We found that, in both cases, micro-droplets spontaneously transferred from the oil phase to the water phase. O/O micro-droplets transformed into oil-in-water micro-droplets, while W/O micro-droplets led to the formation of liposomes.
Citing Articles
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This article has been cited by 12 ACS Journal articles (5 most recent appear below).

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Oriented Reconstitution of a Membrane Protein in a Giant Unilamellar Vesicle: Experimental Verification with the Potassium Channel KcsA
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Miho Yanagisawa, Masayuki Iwamoto, Ayako Kato, Kenichi Yoshikawa, and Shigetoshi OikiJournal of the American Chemical Society2011 Article ASAPWe report a method for the successful reconstitution of the KcsA potassium channel with either an outside-out or inside-out orientation in giant unilamellar vesicles, using the droplet-transfer technique. The procedure is rather simple. First, we prepared ...

Microfluidic Fabrication of Asymmetric Giant Lipid Vesicles
Peichi C. Hu, Su Li, and Noah MalmstadtACS Applied Materials & Interfaces2011 3 (5), 1434-1440Microfluidic Fabrication of Asymmetric Giant Lipid Vesicles
Peichi C. Hu, Su Li, and Noah MalmstadtACS Applied Materials & Interfaces2011 3 (5), 1434-1440We have developed a microfluidic technology for the fabrication of compositionally asymmetric giant unilamellar vesicles (GUVs). The vesicles are assembled in two independent steps. In each step, a lipid monolayer is formed at a water−oil interface. The ...

Dynamic Behavior of Giant Liposomes at Desired Osmotic Pressures
Masae Ohno, Tsutomu Hamada, Kingo Takiguchi and Michio HommaLangmuir2009 25 (19), 11680-11685Dynamic Behavior of Giant Liposomes at Desired Osmotic Pressures
Masae Ohno, Tsutomu Hamada, Kingo Takiguchi and Michio HommaLangmuir2009 25 (19), 11680-11685To apply accurate and uniform osmotic pressures to liposomes, they can be formed using the spontaneous transfer method in solutions with different osmolarities. The majority of liposomes unexpectedly opened large holes (several micrometers in diameter) in ...
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History
- Published In Issue November 21, 2006
- Received July 27, 2006
Revised October 5, 2006
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