Protein Recording Material:  Photorecord/Erasable Protein Array Using a UV-Eliminative Linker

Koji Nakayama, Takashi Tachikawa, and Tetsuro Majima*
The Institute of Scientific and Industrial Research (ISIR), Osaka University Mihogaoka 8-1, Ibaraki, Osaka 567-0047, Japan
Langmuir, 2008, 24 (5), pp 1625–1628
DOI: 10.1021/la703354c
Publication Date (Web): January 23, 2008
Copyright © 2008 American Chemical Society

Abstract

Abstract Image

Protein patterning on solid surfaces is a topic of significant importance in the fields of biosensors, diagnostic assays, cell adhesion technologies, and biochip microarrays. In this letter, we have established a novel, rapid method for the fabrication of a “protein recording material”, which enables us to spatiotemporally regulate the recording, reading, and erasing of a fluorescent protein array as information by a photochemical technique. A photolinker that we synthesized here was used to control the protein array spatiotemporally. The recording process was almost completed after 1 min of photoirradiation to read a clear pattern consisting of a specific protein−ligand complex with high spatiotemporal resolution. The erasing of the protein array was then achieved by photoirradiation onto the entire patterned surface.

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History

  • Published In Issue March 04, 2008
  • Received October 27, 2007
    Revised December 14, 2007

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