Efficient Immobilization and Patterning of Live Bacterial Cells

Zhiyong Suo,† Recep Avci,*† Xinghong Yang,‡ and David W. Pascual‡
Imaging and Chemical Analysis Laboratory, Department of Physics, and Veterinary Molecular Biology, Montana State University, Bozeman, Montana 59717
Langmuir, 2008, 24 (8), pp 4161–4167
DOI: 10.1021/la7038653
Publication Date (Web): March 6, 2008
Copyright © 2008 American Chemical Society
†

 Department of Physics.

,
*

 To whom correspondence should be addressed. E-mail:  avci@physics. montana.edu. Telephone:  406-994-6164. Fax:  406-994-6040.

,
‡

 Veterinary Molecular Biology.

Abstract

Abstract Image

A monolayer of live bacterial cells has been patterned onto substrates through the interaction between CFA/I fimbriae and the corresponding antibody. Patterns of live bacteria have been prepared with cellular resolution on silicon and gold substrates for Salmonella enterica serovar Typhimurium as a model with high specificity and efficiency. The immobilized cells are capable of dividing in growth medium to form a self-sustaining bacterial monolayer on the patterned areas. Interestingly, the immobilized cells can alter their orientation on the substrate, from lying-down to standing-up, as a response to the cell density increase during incubation. This method was successfully used to sort a targeted bacterial species from a mixed culture within 2 h.

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History

  • Published In Issue April 15, 2008
  • Received December 31, 2007
    Revised January 28, 2008

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