Light-Controlled Molecular Shuttles Made from Motor Proteins Carrying Cargo on Engineered Surfaces

Henry Hess,† John Clemmens,† Dong Qin,† Jonathon Howard,‡ and Viola Vogel*†
Department of Bioengineering, University of Washington, Seattle Washington 98195, and Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
Nano Letters, 2001, 1 (5), pp 235–239
DOI: 10.1021/nl015521e
Publication Date (Web): April 24, 2001
Copyright © 2001 American Chemical Society
†

 University of Washington.

,
‡

 Department of Physiology and Biophysics, University of Washington, and Max Planck Institute of Molecular Cell Biology and Genetics.

,
*

 Corresponding author. Fax:  (206) 685-4434. E-mail:  vvogel@ u.washington.edu.

Abstract

Abstract Image

Molecular shuttles have been built from motor proteins capable of moving cargo along engineered paths. We illustrate alternative methods of controlling the direction of motion of microtubules on engineered kinesin tracks, how to load cargo covalently to microtubules, and how to exploit UV-induced release of caged ATP combined with enzymatic ATP degradation by hexokinase to turn the shuttles on and off sequentially. These are the first steps in the development of a tool kit to utilize molecular motors for the construction of nanoscale assembly lines.

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History

  • Published In Issue May 09, 2001
  • Received February 21, 2001

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