Using Nanografting to Achieve Directed Assembly of de novo Designed Metalloproteins on Gold

Martin A. Case,*† George L. McLendon,†‡ Ying Hu,†‡ T. Kyle Vanderlick,‡ and Giacinto Scoles†‡
The Department of Chemistry and Princeton Materials Institute, Princeton University, Princeton, New Jersey 08544
Nano Letters, 2003, 3 (4), pp 425–429
DOI: 10.1021/nl025795h
Publication Date (Web): October 26, 2002
Copyright © 2003 American Chemical Society
*

 Corresponding author. Telephone 609-258-1371; Fax 609-258-6746; E-mail:  macase@princeton.edu

,
†

 The Department of Chemistry, Princeton University.

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‡

 Princeton Materials Institute, Princeton University.

Abstract

Abstract Image

Parallel three-helix bundle metalloproteins incorporating C-terminal thiol groups have been designed to orient vertically on a gold(111) surface. A small area of pre-assembled octadecanethiol was exchanged with the proteins under the action of an AFM tip. The resulting “nanografted” metalloprotein was imaged at low applied force. The measured height of the octadecanethiol monolayer was 2.2(1) nm and the mean height above this of the grafted metalloprotein layer was 3.1(4) nm. Thus the total height of the grafted metalloprotein is 5.3(4) nm. The predicted height of the vertically oriented metalloprotein is 5.2 nm. We take this as good evidence for spatial control of monolayer assembly of three-helix bundles with predictable orientation.

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History

  • Published In Issue April 09, 2003
  • Received September 16, 2002
    Revised Manuscript Received September 30, 2002

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