Macroscopic 2D Networks Self-Assembled from Nanometer-Sized Protein/DNA Complexes
- Maximino Manzanera
- ,
- Daniel J. Frankel
- ,
- Haitao Li
- ,
- Dejian Zhou
- ,
- Andreas Bruckbauer
- ,
- Peter Kreutzmann
- ,
- Jonathan M. Blackburn
- ,
- Chris Abell
- ,
- Trevor Rayment
- ,
- David Klenerman
- , and
- Paul D. Barker
Abstract
We demonstrate the self-assembly of DNA and DNA binding proteins into two-dimensional networks that are then addressable by sending a second protein to a specific recognition site on the DNA network. These networks cover centimeters in area but can be addressed with nanometer precision. This hierarchical self-assembly of specific DNA protein complexes will be the basis for complex positioning of single molecules in two and three dimensions.
†
Department of Chemistry, University of Cambridge.
⊥
These authors contributed equally to this work; M.E.M. performed all molecular biology, protein chemistry, and sample preparation, and D.J.F. provided the detailed AFM characterization of the networks.
‡
Present addresses: T.R. is at the School of Chemistry, University of Birmingham, Edgbaston, Birmingham, B15 2TT, U.K.; D.J.F. is at the School of Natural Sciences, Bedson Building, University of Newcastle upon Tyne, Newcastle upon Tyne, NE1 7RU, U.K.
§
Centre for Nanoscience, University of Cambridge.
‖
University of Western Cape.
*
To whom correspondence should be addressed at department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, U.K.
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