Nanoscale Pipetting for Controlled Chemistry in Small Arrayed Water Droplets Using a Double-Barrel Pipet

Kit T. Rodolfa,† Andreas Bruckbauer,† Dejian Zhou,† Andrew I. Schevchuk,‡ Yuri E. Korchev,‡ and David Klenerman*†
Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, United Kingdom, and Division of Medicine, Imperial College London, Hammersmith Hospital Campus, Du Cane Road, London, W12 0NN, United Kingdom
Nano Lett., 2006, 6 (2), pp 252–257
DOI: 10.1021/nl052215i
Publication Date (Web): January 7, 2006
Copyright © 2006 American Chemical Society
†

 University of Cambridge.

,
‡

 Imperial College London.

,
*

 Corresponding author. E-mail:  dk10012@cam.ac.uk.

Abstract

Abstract Image

We present a new methodology which provides for the miniaturization of one of the most common tools in use in chemistry and biology laboratories todaythe micropipet. We have used glass-fabricated double-barrel nanopipets to controllably produce arrayed water droplets with volumes as small as a few attoliters under an organic layer. We have addressed individual droplets and added controlled amounts of either additional volume or reagents from one of the barrels of the pipet. We demonstrate that this method can be used for miniaturized cell-free protein expression.

View: Full Text HTML | Hi-Res PDF

Tools

History

  • Published In Issue February 08, 2006
  • Received November 10, 2005
    Revised Manuscript Received December 19, 2005

Recommend & Share

Related Content

Other ACS content by these authors: