Prev. Article Next Article Table of Contents

Letter

High Throughput Quantification of Protein Expression of Cancer Antigens in Tissue Microarray Using Quantum Dot Nanocrystals

Supporting Info

Arezou A. Ghazani,^†^‡^§ Jeongjin A. Lee,^‡^§ Jesse Klostranec,^‡^§ Qing Xiang,^‡^§ Ralph S. Dacosta, Brian C. Wilson, Ming S. Tsao,^† and Warren C. W. Chan*^‡^§^#

Division of Applied Molecular Oncology, Ontario Cancer Institute, Toronto, Ontario M5G 2M9, Canada, The Terrence Donnelly Centre for Cellular and Biomolecular Research, Toronto, Ontario M5S 3E1, Canada, Institute of Biomaterials and Biomedical Engineering, University of Toronto, Ontario M5S 3G9, Canada, Department of Medical Biophysics, University of Toronto, Toronto, Ontario M5G 2M9, Canada, Department of Pathology, Princess Margaret Hospital, University Health Network, Toronto, Ontario M5G 2M9, Canada, Department of Materials Science & Engineering, University of Toronto, Toronto, Ontario M5S 3E4, Canada, and Department of Chemistry, University of Toronto, Toronto, Ontario M5S 3H6, Canada

Nano Lett., 2006, 6 (12), pp 2881–2886

DOI: 10.1021/nl062111n

Publication Date (Web): November 17, 2006

†

Division of Applied Molecular Oncology, Ontario Cancer Institute.

‡

The Terrence Donnelly Centre for Cellular and Biomolecular Research.

Institute of Biomaterials and Biomedical Engineering, University of Toronto.

Department of Medical Biophysics, University of Toronto.

Department of Pathology, Princess Margaret Hospital.

Corresponding author. warren.chan@utoronto.ca

Department of Materials Science & Engineering, University of Toronto.

Abstract

We developed and validated a novel method for quantifying protein expression of cancer tumors in an accurate, sensitive, and high throughput format. This technique integrates quantum dots, tissue microarray, optical spectroscopy, and algorithm design for analysis of tumor biopsies. The integration of this method for tissue analysis in the clinic bears potential impact for improving the diagnosis and treatment of cancer.

View: Full Text HTML | Hi-Res PDF