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Letter

Effects of Mechanical Flexion on the Penetration of Fullerene Amino Acid-Derivatized Peptide Nanoparticles through Skin

Supporting Info

Jillian G. Rouse,^†^‡ Jianzhong Yang,^§ Jessica P. Ryman-Rasmussen,^† Andrew R. Barron,^§ and Nancy A. Monteiro-Riviere*^†^‡

Center for Chemical Toxicology Research and Pharmacokinetics, Department of Clinical Sciences, and Department of Biomedical Engineering, North Carolina State University, 4700 Hillsborough Street, Raleigh, North Carolina 27606, and Department of Chemistry and the Richard E. Smalley Institute for Nanoscale Science and Technology, Rice University, Houston, Texas 77005

Nano Lett., 2007, 7 (1), pp 155–160

DOI: 10.1021/nl062464m

Publication Date (Web): December 6, 2006

†

Center for Chemical Toxicology Research and Pharmacokinetics, Department of Clinical Sciences, North Carolina State University.

‡

Department of Biomedical Engineering, North Carolina State University.

Department of Chemistry and the Richard E. Smalley Institute for Nanoscale Science and Technology, Rice University.

To whom correspondence should be addressed. E-mail address: Nancy_Monteiro@ncsu.edu. Telephone: 919-513-6426. Fax: 919-513-6358.

Abstract

Dermatomed porcine skin was fixed to a flexing device and topically dosed with 33.5 mg·mL^-1 of an aqueous solution of a fullerene-substituted phenylalanine (Baa) derivative of a nuclear localization peptide sequence (Baa-Lys(FITC)-NLS). Skin was flexed for 60 or 90 min or left unflexed (control). Confocal microscopy depicted dermal penetration of the nanoparticles at 8 h in skin flexed for 60 and 90 min, whereas Baa-Lys(FITC)-NLS did not penetrate into the dermis of unflexed skin until 24 h. TEM analysis revealed fullerene-peptide localization within the intercellular spaces of the stratum granulosum.

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