Systematic Investigation of the Thermodynamics of HSA Adsorption to N-iso-Propylacrylamide/N-tert-Butylacrylamide Copolymer Nanoparticles. Effects of Particle Size and Hydrophobicity

Stina Lindman,† Iseult Lynch,†‡ Eva Thulin,† Hanna Nilsson,† Kenneth A. Dawson,‡ and Sara Linse*†‡§
Department of Biophysical Chemistry, Chemical Centre, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden, School of Chemistry and Chemical Biology, and Conway Institute, University College Dublin, Belfield, Dublin 4, Ireland
Nano Lett., 2007, 7 (4), pp 914–920
DOI: 10.1021/nl062743+
Publication Date (Web): March 3, 2007
Copyright © 2007 American Chemical Society
†

 Lund University.

,
‡

 School of Chemistry and Chemical Biology, University College Dublin.

,
*

 Corresponding author. E-mail:  Sara.Linse@bpc.lu.se or sara@ fiachra.ucd.ie.

,
§

 Conway Institute, University College Dublin.

Abstract

Abstract Image

Nanoparticles in biological fluids almost invariably become coated with proteins that may confer nanomedical and nanotoxicological effects. Understanding these effects requires quantitative measurements using simple systems. Adsorption of HSA to copolymer nanoparticles of varying hydrophobicity and curvature was studied using ITC, yielding stoichiometry, affinity, and enthalpy changes upon binding. The hydrophobicity was controlled via the co-monomer ratio, N-iso-propylacrylamide/N-tert-butylacrylamide. The most hydrophobic particles become fully covered with a single layer of protein, except at high curvature.

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History

  • Published In Issue April 11, 2007
  • Received November 23, 2006
    Revised Manuscript Received February 10, 2007

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