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Letter
Artificial Photosynthesis in Ranaspumin-2 Based Foam
Abstract
We present a cell-free artificial photosynthesis platform that couples the requisite enzymes of the Calvin cycle with a nanoscale photophosphorylation system engineered into a foam architecture using the Túngara frog surfactant protein Ranaspumin-2. This unique protein surfactant allowed lipid vesicles and coupled enzyme activity to be concentrated to the microscale Plateau channels of the foam, directing photoderived chemical energy to the singular purpose of carbon fixation and sugar synthesis, with chemical conversion efficiencies approaching 96%.
A detailed methods section and supporting experiments as well as controls, supporting figures showing control experiments comparing photoderived ATP production in the bulk, foam, and deflated foam solutions, a demonstration of carbon fixation using RuBisCO RuBP and stock ATP in a bulk aqueous solution, and carbon fixation assay controls, tables summarizing the glucose production and glucose control assays with selected assay components removed and a comparison between bulk solution and the foam for the entire glucose production system, and figures showing glucose assay standard curve and the gene synthesis and protein purification results for Rsn-2, vesicle size information in the form of dynamic light scattering and TEM micrographs, and the fluorescent vesicles in Rsn-2 and T20 foam solutions. This material is available free of charge via the Internet at http://pubs.acs.org.
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