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Letter

High-Resolution Structural Analysis of a DNA Nanostructure by cryoEM

Takayuki Kato^†, Russell P. Goodman^§, Christoph M. Erben^§, Andrew J. Turberfield^§ and Keiichi Namba*^†^‡

Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan, Dynamic Nanomachine Project, ICORP, JST, 1-3 Yamadaoka, Suita, Osaka, 565-0871, Japan, and Clarendon Laboratory, Department of Physics, University of Oxford, Oxford, OX1 3PU, U.K.

Nano Lett., 2009, 9 (7), pp 2747–2750

DOI: 10.1021/nl901265n

Publication Date (Web): June 3, 2009

* To whom correspondence should be addressed. E-mail: keiichi@fbs.osaka-u.ac.jp., †

Osaka University.

, ‡

Dynamic Nanomachine Project, ICORP, JST.

, §

University of Oxford.

Abstract

Many DNA nanostructures have been produced and a wide range of potential applications have been proposed. However, confirmation of accurate 3D construction is particularly challenging. Here, we demonstrate that cryoEM may be exploited to obtain structural information at sufficient resolution to visualize the DNA helix and reveal the absolute stereochemistry of a 7 nm self-assembled DNA tetrahedron. Structural analysis at such high resolution by cryoEM image analysis is unprecedented for any biological molecule of this size.

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History

Published In Issue July 08, 2009
Article ASAPJune 03, 2009
Received: April 21, 2009
Revised: May 24, 2009

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Letter

High-Resolution Structural Analysis of a DNA Nanostructure by cryoEM

Abstract

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History

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