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Imaging Surface Charges of Individual Biomolecules

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Carl Leung*^†, Helen Kinns^‡, Bart W. Hoogenboom^§, Stefan Howorka^‡ and Patrick Mesquida^†

Department of Mechanical Engineering, King’s College London, London WC2R 2LS, England, Department of Chemistry, University College London, London WC1H 0AH, England, and London Centre for Nanotechnology and Department of Physics & Astronomy, University College London, London WC1H 0AH, England

Nano Lett., 2009, 9 (7), pp 2769–2773

DOI: 10.1021/nl9012979

Publication Date (Web): June 11, 2009

* Corresponding author, carl.lsc@gmail.com or carl.leung@kcl.ac.uk., †

Department of Mechanical Engineering, King’s College London.

, ‡

Department of Chemistry, University College London.

, §

London Centre for Nanotechnology and Department of Physics & Astronomy, University College London.

Section:

Biochemical Methods

Abstract

Surface charges play a key role in determining the structure and function of proteins, DNA, and larger biomolecular structures.(1-4) Here we report on the measurement of the electrostatic surface potential of individual DNA and avidin molecules with nanometer resolution using Kelvin probe force microscopy. We also show, for the first time, the surface potential of buffer salts shielding individual DNA molecules, which would not be possible with conventional ensemble techniques.

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