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Construction of a 4 Zeptoliters Switchable 3D DNA Box Origami

†Department of Molecular Biology and Genetics, ‡ Danish National Research Foundation: Centre for DNA Nanotechnology (CDNA) and § Interdisciplinary Nanoscience Center (iNANO), Aarhus University, DK-8000 Aarhus, Denmark
ACS Nano, 2012, 6 (11), pp 10050–10053
DOI: 10.1021/nn303767b
Publication Date (Web): October 2, 2012
Copyright © 2012 American Chemical Society
*Address correspondence to jk@mb.au.dk.

Abstract

Abstract Image

The DNA origami technique is a recently developed self-assembly method that allows construction of 3D objects at the nanoscale for various applications. In the current study we report the production of a 18 × 18 × 24 nm3 hollow DNA box origami structure with a switchable lid. The structure was efficiently produced and characterized by atomic force microscopy, transmission electron microscopy, and Förster resonance energy transfer spectroscopy. The DNA box has a unique reclosing mechanism, which enables it to repeatedly open and close in response to a unique set of DNA keys. This DNA device can potentially be used for a broad range of applications such as controlling the function of single molecules, controlled drug delivery, and molecular computing.

Keywords:

3D DNA origami; dynamic origami; FRET; multiswitchable nanostructure;

Extended materials and methods including designs, further gel, AFM and TEM images, FRET and DLS studies, computer codes, and list of sequences. This material is available free of charge via the Internet at http://pubs.acs.org.

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Article Views: 2,062 Times
Received 17 August 2012
Date accepted 2 October 2012
Published online 2 October 2012
Published in print 27 November 2012
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