Structural Characterization and DPPH Radical Scavenging Activity of an Arabinoglucogalactan from Panax notoginseng Root

Yalin Wu*† and Denong Wang‡
Department of Chemistry, Columbia University, New York, New York 10027, and Department of Genetics, Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, California 94305-5318
J. Nat. Prod., 2008, 71 (2), pp 241–245
DOI: 10.1021/np070323+
Publication Date (Web): January 11, 2008
Copyright © 2008 The American Chemical Society and American Society of Pharmacognosy
* To whom correspondence should be addressed. Tel: +1 347-933-3899 . E-mail: yw2248@columbia.edu. Current address: Columbia University, Department of Ophthalmology, New York, NY 10032.
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†

Columbia University.

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‡

Stanford University.

Abstract

Abstract Image

The structure of an antiradical arabinoglucogalactan (1) from Panax notoginseng roots was determined. This polymeric carbohydrate was obtained through successive phosphate buffer (pH 7.0) extraction after cold-water pretreatment and purification by ion-exchange and gel-filtration chromatography. Monosaccharide analysis, permethylation analysis, NaIO4 and CrO3 oxidations, Smith degradation, graded acid hydrolysis, and IR and NMR experiments indicated that 1 possesses a backbone of (1→3)-linked β-d-galactofuranosyl residues, with branches of α-l-Araf-(1→4)-β-d-Glcp-(1→ residues at O-6. Additionally, 1 exhibited high scavenging activity against DPPH free radicals with a 50% scavenging concentration (SC50) of 11.72 ± 0.91 µg/mL, suggesting that this arabinoglucogalactan is a potential antiradical.

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History

  • Published In Issue February 22, 2008
  • Article ASAPJanuary 11, 2008
  • Received: July 03, 2007

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